November 27, 2017 to November 29, 2017
Mothur Workshop
8:30 am to 4:00 pm
Location
Romulus, Michigan
Contact meeting organizer for details
Event Description
mothur workshop
This workshop is geared towards people with diverse backgrounds so you shouldn't feel like this isn't for you if you are a beginner. I assume no previous computer programming experience. The workshop is an even blend of lecture/discussion and hands on use of mothur with real sequence data. Here is a rough outline of what the workshop schedule will look like (although it is subject to some minor changes)...
Day 1:
- Overview of methods used in microbial ecology
- DNA sequencing technologies (Sanger, 454, Illumina, IonTorrent, PacBio)
- Introduction to mothur, command line interface, and GUI
- Improving sequence quality
- Alignment
Day 2:
- Chimera detection
- Distance calaculations
- OTU identification
- Sequence/OTU classification
Day 3:
- Intro to microbial ecology
- Methods of measuring alpha and beta diversity
- Population level analyses