I'll be sharing three projects, each an attempt to bring new approaches to our study of lung microbiology. I'll show how we're using large animal (pig) models of critical illness to determine why the lung microbiome is altered in sepsis and the acute respiratory distress syndrome. I'll share our early experience using the MinION, a handheld nanopore DNA sequencer, to identify pathogens in pneumonia using real-time metagenomics. Finally, I'll show how we use cage/shipment/vendor variation in mouse microbiota to determine if lung immunity reflects gut or lung bacteria.
Sponsored by the Host Microbiome Initiative