December 17, 2018 to December 19, 2018

Mothur Workshop

9:00 am to 5:00 am


Romulus Michigan

Event Description

mothur workshop

This workshop is geared towards people with diverse backgrounds so you shouldn't feel like this isn't for you if you are a beginner. I assume no previous computer programming experience. The workshop is an even blend of lecture/discussion and hands on use of mothur with real sequence data. Here is a rough outline of what the workshop schedule will look like (although it is subject to some minor changes)...
Day 1:

  • Overview of methods used in microbial ecology
  • DNA sequencing technologies (Sanger, 454, Illumina, IonTorrent, PacBio)
  • Introduction to mothur, command line interface, and GUI
  • Improving sequence quality
  • Alignment

Day 2:

  • Chimera detection
  • Distance calaculations
  • OTU identification
  • Sequence/OTU classification

Day 3:

  • Intro to microbial ecology
  • Methods of measuring alpha and beta diversity
  • Population level analyses