Immune-mediated control of Toxoplasma and Salmonella in human cells

Seminar Details
Thursday, February 25, 2021 - 12:00pm to 1:00pm


Eva Marie Frickel
Group Leader: Host-Toxoplasma Interaction Laboratory,
University of Birmingham
The Medical Research Council's National Institute for Medical Research
The Francis Crick Institute


Hosted by: Vern Carruthers

Microbiology and Immunology Seminar Series

Abstract: Infection with intracellular pathogens such as the parasite Toxoplasma gondii and the bacterium Salmonella typhimurium generates the cytokine interferon gamma (IFN). IFN stimulates cells to upregulate host defense proteins, amongst them the interferon-inducible guanylate binding proteins (GBPs). GBPs promote cell-intrinsic defense by targeting pathogens and pathogen-containing vacuoles for membrane-disruption that releases microbial molecules to induce host cell death. GBP1 mediates pyroptosis or atypical apoptosis of Salmonella- or Toxoplasma-infected human macrophages, respectively. We have developed an artificial intelligence-driven image analysis pipeline (HRMAn, Host Response to Microbe Infection) enabling us to conduct high throughput single-cell analysis of these host defense mechanisms. Combined with mechanistic cellular studies and unbiased mass spectrometry approaches, we are addressing the host cell intrinsic defense pathways driven by GBPs in response to these two evolutionarily divergent pathogens.