Visualizing Bacterial Physiology at High Resolution using Single-Molecule Tracking and Lattice-Light Sheet Microscopy

Thursday, February 13, 2020 - 4:00pm to 5:30pm
Andreas Gahlmann, Ph.D.
Assistant Professor
Department of Chemistry
Department of Molecular Physiology & Biological Physics
Center for Cell and Membrane Physiology
University of Virginia

Our lab develops new imaging approaches for visualizing bacterial physiology in relevant contexts: We use live-cell single-molecule localization microscopy and lattice-light sheet microscopy to access 3D spatial and temporal information with high resolution. At molecular and cellular length scales, our research focuses on understanding how Gram-negative bacterial pathogens inject effector proteins into the cytosol of eukaryotic host cells using the Type 3 Secretion System (T3SS) â a 7 MDa multi-protein complex that spans two, and sometimes three cellular membranes.