- New Rates In Effect Starting June 1, 2016 -
The MSL sequencing group is capable of isolating DNA from tissues, swabs and multiple environmental sources through an automated system. The lab is home to a BioMek FX (Beckman) and an epMotion (Eppendorf) liquid handling robot that are capable of extracting nucleic acids from as many as 96 samples concurrently. We frequently use Isolation kits, such as the PowerSoil and the PowerMag isolation kits, both supplied by MoBio. Sequencing is performed using the Illumina (MiSeq) platform. Minimum sequencing jobs are one quarter run for University of Michigan clients and one full run for external clients.
Guidelines for DNA submission:
- Recommended DNA Isolation kits: MoBio PowerMicrobiome, MoBio PowerSoil, MoBio PowerMag, Roche MagnaPure, Qiagen spin columns. Other kits may be used if high quality DNA is extracted.
- Please submit 15 µL of isolated DNA in a full skirted twin tec PCR plate (Eppendorf cat #951020401) sealed with a sterile aluminum foil (VWR cat #89049-034).
- Expected DNA concentrations should be in the range of 1-100 ng/µL. If <1 ng/µL, please indicate samples as "low bio-mass" on request form.
- Samples should be filled column-wise.
- Please provide an electronic copy plate map using this template: Click here. NOTE: For MiSeq runs, sample ID must be 1 word containing NO special characters (including no underlines or dashes). Each name MUST be unique, even if on different plates.
- Ideally, the customer should test DNA for amplification ahead of submission. If you would like to order generic primers, please contact the lab at MSMBLCORE@umich.edu.
- For proper shipping protocol please refer to these guidelines: Click here.
- Sample form for Illumina sequencing: MiSeq Request
Microbial Systems Molecular Biology Laboratory Host Microbiome Initiative University of Michigan Medical School Internal Medicine/Infectious Diseases 1500 MSRB1 1150 W. Medical Center Dr. Ann Arbor, MI 48109-5666 Contact For further information about sequencing services, please contact: April Cockburn email@example.com 734-615-2958 Michael Dority firstname.lastname@example.org 734-764-3016
Download FASTA file for the V4 region of the mock community (ZymoBIOMICS Microbial Community DNA Standard)