December 17, 2018 to December 19, 2018
Mothur Workshop
9:00 am to 5:00 am
Location
Romulus Michigan
Event Description
mothur workshop
This workshop is geared towards people with diverse backgrounds so you shouldn't feel like this isn't for you if you are a beginner. I assume no previous computer programming experience. The workshop is an even blend of lecture/discussion and hands on use of mothur with real sequence data. Here is a rough outline of what the workshop schedule will look like (although it is subject to some minor changes)...
Day 1:
- Overview of methods used in microbial ecology
- DNA sequencing technologies (Sanger, 454, Illumina, IonTorrent, PacBio)
- Introduction to mothur, command line interface, and GUI
- Improving sequence quality
- Alignment
Day 2:
- Chimera detection
- Distance calaculations
- OTU identification
- Sequence/OTU classification
Day 3:
- Intro to microbial ecology
- Methods of measuring alpha and beta diversity
- Population level analyses