Microbial Community Profiling:
We now offer three different ways to profile microbial communities:
- Standard 16S - Our standard 16S protocol (Kozich et al., 2013) targets and amplifies the V4 region of bacterial and archaeal 16S rRNA-encoding genes.
- 16S EMP - A slightly modified set of primers designed by the Earth Microbiome Project (EMP) that also targets the V4 region with added degeneracies to be more inclusive of bacterial and archaeal species found in some environments (Walters et al., 2015).
- Fungal ITS - Primers targeting the fungal Internally Transcribes Spacer (ITS) region for amplification of a broad range of mycological taxa in complex community samples (Taylor et al., 2016).
Community profiling can occur on purified DNA samples from a variety of sources, including blood, stool, saliva and soil. We accept samples in a 96-well format, with up to 94 samples and 2 blank wells per plate. Purified nucleic acids can be quantified at the Core, or we will process the samples as described in our Nucleic Acid Extraction service. Libraries are cleaned, normalized, and undergo quality control before sequencing on the Illumina MiSeq platform. Output data (ex: FASTQ files) can be returned to our clients via Illumina’s BaseSpace Sequence Hub or on a USB flash drive.
If you are ready to submit samples for community profiling, we process all submissions through our MiCores website.
If you have further questions, please refer to the Frequently Asked Questions page.