Microbial Community Profiling:
The V4 region of the 16S rRNA-encoding gene is amplified from extracted DNA using the barcoded dual-index primers developed by Kozich et al. (1). Samples are amplified, normalized and sequenced on the MiSeq. Up to 4 plates/376 samples + 8 controls can be run concurrently using a single sequencing kit. For clients with fewer samples we can utilize the Illumina Nano kit to run up to 94 samples + 2 controls. The client can expect a workbook outlining the conditions used to generate the library and metrics of the sequencing run. Data is delivered via Basespace or USB (supplied by us).
DNA extraction: We can accept purified DNA or we can run the extractions for you. Clients who require there samples to be extracted prior to sequencing should refer to our Nucleic Acid Extraction page.
16S rDNA sequencing: Clients will be charged for a full run (4 plates/376 samples + 8 controls) regardless of whether they submit less than 4 plates unless the MiSeq Nano kit is selected on the submission form. The Nano kit is a good option for clients with single plates. This route offers fewer reads/sample, but enough coverage for data analysis.
Data delivered by the Microbiome Core will be backed up and available for six months. While these data may be available beyond the six month window, we cannot guarantee having information related to your project after this period.
We ask that all publications and presentations of research results supported by the Microbiome Core contain the following (or equivalent) acknowledgement: "This research was supported by work performed by The University of Michigan Microbiome Core"
Please let us know as soon as you have an article accepted for publication, poster, or meeting presentation which has received Microbiome Core support. Early notification helps us to track the activities being supported by the Microbiome Core. Submit your notifications by email to firstname.lastname@example.org. Be sure to include a copy of the presentation abstract or complete accepted article.
General Contact for all technical and billing questions: email@example.com
Chris Blair firstname.lastname@example.org 734-615-2958
Michael Dority email@example.com
- Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. 2013. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microbiol 79:5112–5120. 10.1128/AEM.01043-13.