mothur workshop

This workshop is geared towards people with diverse backgrounds so you shouldn't feel like this isn't for you if you are a beginner. I assume no previous computer programming experience. The workshop is an even blend of lecture/discussion and hands on use of mothur with real sequence data. Here is a rough outline of what the workshop schedule will look like (although it is subject to some minor changes).

Day 1:

• Overview of methods used in microbial ecology
• DNA sequencing technologies (Sanger, 454, Illumina, IonTorrent, PacBio)
• Introduction to mothur, command line interface, and GUI
• Improving sequence quality
• Alignment

Day 2:

• Chimera detection
• Distance calculations
• OTU identification
• Sequence/OTU classification

Day 3:

• Intro to microbial ecology
• Methods of measuring alpha and beta diversity
• Population level analyses